Question Given amino acids of Aspartate, Leucine, Lysine, Se

Question Given amino acids of Aspartate, Leucine, Lysine, Serine and Amide forming pentapeptide -Which one would lower the PI of peptide mostly (explain) -Overall Charge at pH thirteen -How many charged groups would it have at pH one and what are they -Would it be able to be detected by absorbance at 290 nm? explain Please help me answer the questions as much as you could. Thank you ! These are self study reference purpose

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